Composite

Part:BBa_K1362109:Design

Designed by: Jan Gleixner   Group: iGEM14_Heidelberg   (2014-10-09)


RBS + NpuDnaE N&C-Intein coexpression RFC[105] nonsplicing assembly construct (with His6)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 826
    Illegal AgeI site found at 938
    Illegal AgeI site found at 2430
    Illegal AgeI site found at 2542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1115
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 40
    Illegal BsaI.rc site found at 1644


Design Notes

As less expression of the N intein was observed in a previous experiment(data not shown) the N construct was cloned before the C part on the polycystronic transcript.


Source

This part was cloned via standard BB Assembly from BBa_K1362102 BBa_K1362103. See their respective sources for more information.


References